Influence of culture media on the Kirby–Bauer method for testing antibacterial activity against Pseudomonas aeruginosa (HO80)

  • Yacine Daoudi
  • Smail Daoudi
  • Noureddine Bouras
  • Mohamed Kraimat
  • Sid Ahmed Saadi
  • Saïd Belghit
  • Abdellah Kemassi
  • Salim Mokrane
  • Michael D. Holtz
  • M’hammed Bouallala
Keywords: Culture medium; Antibiotic; Pseudomonas aeruginosa HO80; Antibiogram; Kirby–Bauer method


We investigated in this study the effect of different culture media on antibiotic susceptibility testing of Pseudomonas aeruginosa HO80 with
the Kirby–Bauer (agar-diffusion) method by measuring the diameters of inhibition zones. We observed that the generally used culture
medium, Mueller-Hinton agar (MHA*), could be replaced by many other culture media, such as Hektoen agar (HKA*), Bennett’s agar (BAM)
and Trypticase Soy Agar (TSA*) media in order to realize antibiograms used in medical and research laboratories for testing Pseudomonas